Determination of the Minimal Inhibitory Concentration (MIC) using E-test

MIC is defined as the lowest concentration of an antimicrobial that will inhibit the visible growth of a microorganism (fungi and yeast) representing a quantitative estimate of the susceptibility of an organism to a certain antimicrobial agent. E-test consists of a predefined, continuous, and exponential gradient of antibiotic concentrations immobilized along plastic test strips which, placed on the surface of agar plate, can release the agent gradually. After the incubation and the microorganism's growth, the point at which the drop-shaped inhibition zone intersects the graded test strip is defined as the MIC of the antibiotic.  A reference standard is used for the quality check of reagents, media and inoculum.

STUDY DESIGN
The MIC value is  determined by agar diffusion procedure using E-TEST in which specify the name of substance, the number and the name of strains, the number and the name of the reference strain. 3 replicates are carried out and M
IC value is expressed as µg/mL.

Study includes GLP management and reporting (if required)

REFERENCES AND GUIDELINES
Antimicrobial Susceptibility Testing 9302553C - en - 2012/01 and Etest® for MIC determination of antifungal agents (AB BIODISK)

 

 

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Simona Coranelli
E-mail: scoranelli@biotecnologiebt.it
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