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The parasitic wasp Aphidius rhopalosiphi was chosen as non-target arthropod (NTA) standard specie since it proved to be the most sensitive in a range of experiments carried out on different species of NTA with many different Plant Protection Products.
Parasitoids adults, less than 48 hours old, are exposed to different concentrations of the test substance applied on glass plate (laboratory test) or on natural surface as plants or plants parts by a calibrated spray equipment, in order to evaluate the effects in terms of mortality over 48 hours exposure period and sub-lethal effects in terms of reproduction capacity of surviving females (10-12 days).
Adults of Aphidius rhopalosiphi
Range finding is carried out with at least 3 application rates of the test substance and 1 untreated control (3 replicates/treatment or control) with 10 adults/replicate.
Limit test includes 1 application rate of the test substance, 1 untreated control and 1 toxic reference treatment (5 replicates/treatment or control) with 10 adults/replicate.
Rate-response test includes at least 5 application rates of the test substance, 1 untreated control and 1 toxic reference treatment (3 replicates/treatment or control) with 10 adults/replicate.
In order to evaluate the duration of the effects of the test substance and the recovery capacity of the insects it is possible to carry out an extended laboratory test with aged residues of the test substance.
The plants are treated in semi-field conditions and the leaves of the treated plants, after an adequate aging time, are moved to laboratory to perform the standard test as previously described.
Mortality: LR50/NOER at 48 hours after treatment.
Reproduction capacity of the surviving females: ER50/NOER.
Study includes GLP management and reporting.
REFERENCES AND GUIDELINES
IOBC/WPRS Guidelines (Mead-Briggs et al., 2000).
Guideline of the ring-testing group (Mead-Briggs et al., 2009).
ESCORT I Guidance Document (Barret K.L. et al., eds. 1994).
ESCORT II Guidance Document (Candolfi et al., eds. 2001).