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OECD TG 214: Honeybees, Acute Contact Toxicity test

Agriculture provides habitat and forage for pollinating insects such as honey bees.
As well as producing honey and a variety of hive products, honey bees perform the ecosystem service of pollination which allows plants to reproduce, provides the fruits and seeds.The use of plant protection product in agriculture leads to exposure of honey bees to pesticide residues. The exposure via contact occurs while honey bees are feeding from the treated crops, from the weeds in treated field, from plants in field margins and from the adjacent crops where the liquid or solid particles resulting from spray/dust drift might be present.

Adult worker honeybees are exposed to the test substance dissolved in appropriate carrier, by direct application to the thorax (droplets) in order to evaluate the mortality and sublethal effects. Mortality and sublethal effects are assessed at 4, 24 and 48 hours after the treatment. If an extention is needed, further observations are carried out at 72 and 96 hours after the beginning of the test.

TEST SYSTEM
Adult honey bees of Apis mellifera L.



STUDY DESIGN

Range finding test includes at least 3 doses of the test substance and 1 untreated control, at least 2 replicates for treatment and for the control with 10 bees/replicate.
Limit test is carried out with 1 dose of the test substance, 1 toxic reference substance and 1 untreated control, 5 replicates for treatment and for the control with 10 bees/replicate.
Dose-response test includes at least 5 doses of the test substance, 1 toxic reference substance and 1 untreated control,  3 replicates for treatment and for the control with 10 bees/replicate.

ENDPOINTS
Mortality: LDx/NOED and behavioural abnormalities are recorded.
Study includes GLP management and reporting.

REFERENCES AND GUIDELINES
OECD Guideline fo Testing of Chemicals, No. 214 (21st September 1998) - Honeybees, Acute contact toxicity test.

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Scientific Contact

Monica Colli
E-mail: mcolli@biotecnologiebt.it
Phone: +39 075 895 0045 - Ext. 260

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Guidelines

OECD TG 214