Find your test

SELECT TEST

Can't find the test you need? Go to the adavnced reserch

ASK FOR
INFORMATION
DONWLOAD
OUR BROCHURE

OECD TG 232: Collembolan Reproduction test in soil

Soil-dwelling Collembola are ecologically relevant species for ecotoxicological testing.
Collembolans are hexapods with a thin exoskeleton highly permeable to air and water, and represent arthropod species with a different route and a different rate of exposure compared to earthworms and enchytraeids.
Synchronized female of Folsomia candida, 9-12 days old, are exposed to the test substance incorporated into the artificial soil by an appropriate carrier or sprayed on the soil surface in order to evaluate the lethal and reproduction effects. Mortality and reproduction performance are assessed after 4 weeks of exposure.

TEST SYSTEM
Folsomia candida
A toxic reference substance is tested once a year to check the sensitivity of the organisms.



STUDY DESIGN

Range finding test lasts for 3 weeks and it includes 5 concentrations of the test substance and 1 untreated control (2 replicates/treatment or control) with 10 females/replicate
Limit test is carried out with 1 concentration of the test substance and 1 untreated control (8 replicates/treatment or control) with 10 females/replicate
Rate-response test for NOEC/LOEC evaluation includes at least 5 concentrations of the test substance and 1 untreated control (4 replicates/treatment and 8 replicates for the control) with 10 females/replicate.
Rate-response test for ECx evaluation includes 12 concentrations of the test substance and 1 untreated control (at least 2 replicates/treatment and 6 replicates for the control) with 10 females/replicate.
Rate-response test for ECx and NOEC/LOEC evaluation is performed with 8 concentrations of the test substance and 1 untreated control (4 replicates/treatment and 8 replicates for the control) with 10 females/replicate.
                                                                
ENDPOINTS
Mortality: LCx  or LOEC/NOEC after 4 weeks of exposure.
Reduction in reproduction: ECx or NOEC/LOEC after 4 weeks of exposure.
Study includes GLP management and reporting.

REFERENCES AND GUIDELINES
OECD Guideline fo Testing of Chemicals, No. 232 (29th July 2016) - Collembolan reproduction test in soil.

ARE YOU INTERESTED ON THIS STUDY/TEST?
CLICK HERE AND ASK NOW FOR MORE INFORMATION!

Scientific Contact

Monica Colli
E-mail: mcolli@biotecnologiebt.it
Phone: +39 075 895 0045 - Ext. 260

Business contact

E-mail: marketing@biotecnologiebt.it

Guidelines

OECD TG 232