OECD TG 238: Sediment-free Myriophyllum spicatum Toxicity test

Myriophyllum spicatum (aquatic plant in house breeding at B.T.) is cultured in Modified Andrews' Medium. Fresh lateral branches from pre-culture shortened to 2.5 cm from base are assigned randomly to the test vessels. The test, under static or semi-static conditions, ends after 14 days of exposure to different concentrations of the test substance. Total shoot length and main shoot length, fresh and dry weight are measured.

TEST SYSTEM
Myriophyllum spicatum
A toxic reference substance is tested twice a year to check the sensitivity of the organisms.



STUDY DESIGN

Range finding test includes from 5 to 7 concentrations of the test substance and 1 untreated control (5 replicates/concentration or control) with one 2,5 cm lateral branch with apical meristem and one end/replicate.
Limit test is carried out with 1 concentration (100 mg/L or at solubility limit) of the test substance and 1 untreated control (10 replicates/concentration and 20 replicates for the control)  with one 2,5 cm lateral branch with apical meristem and one end/replicate.
Dose-response test includes from 5 to 7 concentrations of the test substance and
1 untreated control (5 replicates/concentration and 10 replicates for the control)  with one 2,5 cm lateral branch with apical meristem and one end/replicate.
Analytical checks, to verify the exposure concentrations in the medium, are performed  for static test at time 0 and at 14 days on control and on each test substance sample.
For semi-static test the analysis are carried out at time 0, at 7  days (in spent and fresh solutions) and at 14 days on control and on each test substance sample.
                                                                                    
The analysis are performed using a method validated according to SANCO/825/00 rev. 8.1 and SANCO/3029/99 rev. 4 guidance documents.

ENDPOINTS
Specific growth rate and yield for each measured variable: EC50 at 14 days as the main endpoint and as other endpoints: EC10, EC20, LOEC/NOEC.

Study includes GLP management and reporting.

REFERENCES AND GUIDELINES
OECD Guideline fo Testing of Chemicals, No. 238 (26th September 2014) - Sediment-free Myriophyllum spicatum toxicity test.
SANCO/825/00 rev. 8.1 (16/11/2010) - Guidance document on pesticide residue analytical methods.
SANCO/3029/99 rev. 4 (11/07/2000) - Residues: guidance for generating and reporting methods of analysis in support of pre-registration data requirements for Annex II (Part A, section 4) and Annex III (Part A, section 5) of Directive 91/414.

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Scientific Contact

Sabrina Mantilacci
E-mail: mantilacci@biotecnologiebt.it
Phone: +39 075 895 0045 - Ext. 268

Business contact

E-mail: marketing@biotecnologiebt.it

Guidelines

OECD TG 238